Article
In vitro evidence that UV-induced frameshift and substitution mutations at T tracts are the result of misalignment-mediated replication past a specific thymine dimer.
Biochemistry - 14 Apr 1992
Wang C I, Taylor J S
Abstract excerpt
A previous study of UV-induced (254 nm) mutations in the lacI gene of Escherichia coli found that frameshift mutations accounted for about 35% of the observed mutations and that these mutations occurred predominantly at An.Tn sequences [Miller, J.H. (1985) J. Mol. Biol. 182, 48-65]. Because An.Tn sequences are hotspots for cis-syn thymine dimer formation [Brash, D.E., & Haseltine, W. A. (1982) Nature 298,...
Topics
- Autoradiography
- Base Sequence
- DNA Replication
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Frameshift Mutation
- Molecular Sequence Data
- Mutation
- Pyrimidine Dimers
- Templates, Genetic