Article
Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons.
Clinical chemistry - 1 Jul 2004
Liew Michael, Pryor Robert, Palais Robert, Meadows Cindy, Erali Maria, Lyon Elaine, Wittwer Carl
Abstract excerpt
BACKGROUND: High-resolution melting of PCR amplicons with the DNA dye LCGreen I was recently introduced as a homogeneous, closed-tube method of genotyping that does not require probes or real-time PCR. We adapted this system to genotype single-nucleotide polymorphisms (SNPs) after rapid-cycle PCR (12 min) of small amplicons (</=50 bp). METHODS: Engineered plasmids were used to study all possible SNP base changes....
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