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Article

Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis.

Clinical chemistry - 1 Oct 2004

Reed Gudrun H, Wittwer Carl T

Abstract excerpt

BACKGROUND: Screening for heterozygous sequence changes in PCR products, also known as "mutation scanning", is an important tool for genetic research and clinical applications. Conventional methods require a separation step. METHODS: We evaluated the sensitivity and specificity of homogeneous scanning, using a saturating DNA dye and high-resolution melting. Heterozygous single-nucleotide polymorphism (SNP)...

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PMID: 15308590
DOI: 10.1373/clinchem.2003.029751

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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