Article
Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye.
Clinical chemistry - 1 Aug 2004
Zhou Luming, Myers Alexander N, Vandersteen Joshua G, Wang Lesi, Wittwer Carl T
Abstract excerpt
BACKGROUND: Homogeneous PCR methods for genotyping usually require fluorescently labeled oligonucleotide probes. Amplicon melting with the DNA dye LCGreen I was recently introduced as a closed-tube method of genotyping that does not require probes or real-time PCR. However, some single-nucleotide polymorphisms (SNPs) could not be completely genotyped without addition of a known genotype, and high-resolution...
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