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Article

Multiplex amplicon genotyping by high-resolution melting.

Journal of biomolecular techniques : JBT - 1 Jul 2009

Seipp Michael T, Durtschi Jacob D, Voelkerding Karl V, Wittwer Carl T

Abstract excerpt

High-resolution amplicon melting is a simple method for genotyping that uses only generic PCR primers and a saturating DNA dye. Multiplex amplicon genotyping has previously been reported in a single color, but two instruments were required: a carousel-based rapid cycler and a high-resolution melting instrument for capillaries. Manual transfer of capillaries between instruments and sequential melting of each...

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Identifiers

PMID: 19568454
PMCID: PMC2700465

Genes

MTHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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