Allele-specific extension allows base-pair neutral homozygotes to be discriminated by high-resolution melting of small amplicons.

doi:10.1016/j.ab.2010.06.043

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Article

Allele-specific extension allows base-pair neutral homozygotes to be discriminated by high-resolution melting of small amplicons.

Analytical biochemistry - 1 Nov 2010

Cai Yanning, Yuan Yanpeng, Lin Qingling, Chan Piu

Abstract excerpt

Not all single-nucleotide polymorphisms (SNPs) can be determined using high-resolution melting (HRM) of small amplicons, especially class 3 and 4 SNPs. This is due mainly to the small shift in the melting temperature (Tm) between two types of homozygote. Choosing rs1869458 (a class 4 SNP) as a sample, we developed a modified small amplicon HRM assay. An allele-specific extension (ASE) primer, which ended at an...

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PMID: 20599636
DOI: 10.1016/j.ab.2010.06.043

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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