Article
High-throughput SNP genotyping by single-tube PCR with Tm-shift primers.
BioTechniques - 1 Dec 2005
Wang Jun, Chuang Karen, Ahluwalia Mandeep, Patel Sarika, Umblas Nanette, Mirel Daniel, Higuchi Russell, Germer Soren
Abstract excerpt
Despite many recent advances in high-throughput single nucleotide polymorphism (SNP) genotyping technologies, there is still a great need for inexpensive and flexible methods with a reasonable throughput. Here we report substantial modifications and improvements to an existing homogenous allele-specific PCR-based SNP genotyping method, making it an attractive new option for researchers engaging in candidate gene...
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