Article
Nucleotide extension genotyping by high-resolution melting.
The Journal of molecular diagnostics : JMD - 1 Nov 2010
Liew Michael, Wittwer Carl, Voelkerding Karl V
Abstract excerpt
One limitation of small amplicon melting is the inability to genotype certain nearest-neighbor symmetric variations without manipulating the sample. We have developed a method for these exceptions: a high-resolution melting single nucleotide extension assay. Single nucleotide extension was perfor...
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