Article
Melting curve analysis of SNPs (McSNP): a gel-free and inexpensive approach for SNP genotyping.
BioTechniques - 1 Feb 2001
Akey J M, Sosnoski D, Parra E, Dios S, Hiester K, Su B, Bonilla C, Jin L, Shriver M D
Abstract excerpt
High-throughput methods for assaying DNA variation require two important steps: (i) discriminating the variation and (ii) detecting the signal. In this report, we describe a novel SNP genotyping method that we refer to as melting curve analysis of SNPs (McSNP). McSNP combines a classic approach for discriminating alleles, restriction enzyme digestion, with a more recent method for detecting DNA fragments, melting...
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