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Article

Quadruplex genotyping of F5, F2, and MTHFR variants in a single closed tube by high-resolution amplicon melting.

Clinical chemistry - 1 Jan 2008

Seipp Michael T, Pattison David, Durtschi Jacob D, Jama Mohamed, Voelkerding Karl V, Wittwer Carl T

Abstract excerpt

BACKGROUND: Multiplexed amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, asymmetric PCR, or allele-specific PCR; however, correct differentiation of homozygous mutant and wild-type samples by melting temperature (T(m)) analysis requires high-resolution melting analysis and controlled reaction conditions. METHODS: We designed 4 amplicons bracketing the F5...

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Source

Identifiers

PMID: 17981920
DOI: 10.1373/clinchem.2007.097121

Genes

MTHFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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