Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes.

Clinical chemistry - 1 Mar 2003

Gundry Cameron N, Vandersteen Joshua G, Reed Gudrun H, Pryor Robert J, Chen Jian, Wittwer Carl T

Abstract excerpt

BACKGROUND: Common methods for identification of DNA sequence variants use gel electrophoresis or column separation after PCR. METHODS: We developed a method for sequence variant analysis requiring only PCR and amplicon melting analysis. One of the PCR primers was fluorescently labeled. After PCR, the melting transition of the amplicon was monitored by high-resolution melting analysis. Different homozygotes were...

Read the complete abstract on PubMed

Topics

Base Sequence
Benzothiazoles
Blood Proteins
Cystic Fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator
DNA

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Diamines

Fluorescent Dyes

Genotype

Globins

Heteroduplex Analysis

Heterozygote

High-Temperature Requirement A Serine Peptidase 2

Homozygote

Humans

Mitochondrial Proteins

Oligonucleotide Probes

Organic Chemicals

Source

Identifiers

PMID: 12600951
DOI: 10.1373/49.3.396

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes.PMID 12600951Read ↗

High-resolution genotyping by amplicon melting analysis using LCGreen.Semantic proximity Read ↗

Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons.Semantic proximity Read ↗

Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye.Semantic proximity Read ↗

Unlabeled oligonucleotides as internal temperature controls for genotyping by amplicon melting.Semantic proximity Read ↗

Quadruplex genotyping of F5, F2, and MTHFR variants in a single closed tube by high-resolution amplicon melting.Semantic proximity Read ↗

Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis.Semantic proximity Read ↗

High-resolution melting analysis of single nucleotide polymorphisms.Semantic proximity Read ↗

High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution.Semantic proximity Read ↗

Genotyping by high-resolution melting analysis.Semantic proximity Read ↗

Scanning the cystic fibrosis transmembrane conductance regulator gene using high-resolution DNA melting analysis.Semantic proximity Read ↗

High resolution melting analysis for gene scanning.Semantic proximity Read ↗

Automated DNA extraction, quantification, dilution, and PCR preparation for genotyping by high-resolution melting.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.