Induction of transition and transversion mutations during random mutagenesis PCR by the addition of 2-hydroxy-dATP.

Abstract excerpt

A 2-substituted purine nucleotide analog, 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), was added to a PCR mixture, to examine its mutagenic potential. The 2-OH-dATP enhanced the total mutation frequency. Interestingly, 2-OH-dATP induced both transition and transversion mutations, including A:T-->G:C, A:T-->C:G and G:C-->T:A mutations. In contrast, other 2-substituted purine nucleotide analogs,...

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