Article

Type II restriction of 2-aminoadenine (dZ) modified DNA and production of dZ-modified plasmid in <i>E. coli</i>

2023-10-17

Abstract excerpt

The modified DNA base 2,6 aminopurine (2-aminoadenine, (d)Z base) was originally found in phages to counteract host encoded restriction systems. However, only a limited number of restriction endonucleases (REases) have been tested on dZ-modified DNA. Herein we report the results of 147 REases activity on dZ-modified PCR DNA. Among the enzymes tested, 53.1% are resistant or partially resistant, and 46.9% are sensit...

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Literature Corpus work: 8ea3083b-d87a-5fea-abb3-4a2c5fb09ae7
DOI: 10.1101/2023.10.17.562781

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