Article
2-Hydroxy-2'-deoxyadenosine 5'-triphosphate enhances A.T --> C.G mutations caused by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate by suppressing its degradation upon replication in a HeLa extract.
Biochemistry - 5 Jun 2007
Satou Kazuya, Kasai Hiroshi, Masutani Chikahide, Hanaoka Fumio, Harashima Hideyoshi, Kamiya Hiroyuki
Abstract excerpt
The coexistence effects of multiple kinds of oxidized deoxyribonucleotides were examined using an SV40 origin-dependent in vitro replication system with a HeLa extract. Oxidized dGTP and dATP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP) and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), were used in this study. The mutation frequency synergistically increased when the two oxidized...
Topics
- Adenosine Triphosphate
- Cell Extracts
- Cell-Free System
- Cells, Cultured
- DNA Mutational Analysis
- DNA Repair Enzymes
- DNA Replication
- Deoxyguanine Nucleotides
- Guanosine Triphosphate
- HeLa Cells