Article
Induction of chromosomal gene mutations in Escherichia coli by direct incorporation of oxidatively damaged nucleotides. New evaluation method for mutagenesis by damaged DNA precursors in vivo.
The Journal of biological chemistry - 1 May 1998
Inoue M, Kamiya H, Fujikawa K, Ootsuyama Y, Murata-Kamiya N, Osaki T, Yasumoto K, Kasai H
Abstract excerpt
We have developed a new strategy for the evaluation of the mutagenicity of a damaged DNA precursor (deoxyribonucleoside 5'-triphosphate) in Escherichia coli. 8-Hydroxydeoxyguanosine triphosphate (8-OH-dGTP) and 2-hydroxydeoxyadenosine triphosphate (2-OH-dATP) were chosen for this study because th...
Topics
- Bacterial Proteins
- Base Sequence
- Chromosomes, Bacterial
- DNA Damage
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Evaluation Studies as Topic
- Lac Repressors
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Nucleic Acid Precursors