Article
Substitution and deletion mutations induced by 2-hydroxyadenine in Escherichia coli: effects of sequence contexts in leading and lagging strands.
Nucleic acids research - 15 Jan 1997
Kamiya H, Kasai H
Abstract excerpt
To evaluate the mutation frequency and the mutation spectrum of 2-hydroxyadenine (2-OH-Ade), an oxidative DNA lesion, the modified base was site-specifically incorporated into a unique restriction enzyme site (SalI, GTCGA*C or AflII, CTTA*AG where A* represents 2-OH-Ade) in single- and double-str...
Topics
- DNA
- DNA Primers
- Escherichia coli
- Genetic Vectors
- Guanine
- Mutagenesis, Site-Directed
- Mutation
- Point Mutation
- Transfection