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Article

Induction of various mutations during PCRs with manganese and 8-hydroxy-dGTP.

Biological & pharmaceutical bulletin - 1 Apr 2007

Kamiya Hiroyuki, Ito Mana, Harashima Hideyoshi

Abstract excerpt

To induce various mutations randomly, PCRs with Mn2+ and with a mutagenic deoxyribonucleotide, 8-hydroxy-dGTP (8-OH-dGTP), were performed. Mutations were induced by deoxyribonucleotide imbalance plus 500 microM Mn2+ in the Mn2+-PCR, and the amplified DNA was inserted into a plasmid. The plasmid library obtained from the transformed bacterial cells was then used as the template in the next PCR, which was done with...

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PMID: 17409535
DOI: 10.1248/bpb.30.842

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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