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Article

Kinetics of ATP binding to the origin recognition complex of Saccharomyces cerevisiae.

The Journal of biological chemistry - 21 Nov 2003

Makise Masaki, Takenaka Hitomi, Kuwae Wakako, Takahashi Naoko, Tsuchiya Tomofusa, Mizushima Tohru

Abstract excerpt

Origin recognition complex (ORC), a candidate initiator of chromosomal DNA replication in eukaryotes, binds specifically to ATP through two of its subunits (Orc1p and Orc5p). In this study, we investigated the kinetics of ATP binding to ORC by a filter binding assay. The Kd values for the ATP of wild-type ORC and ORC-1A (mutant ORC containing Orc1p with a defective Walker A motif) were less than 10 nm, suggesting...

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PMID: 12966094
DOI: 10.1074/jbc.M307392200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Kinetics of ATP binding to the origin recognition complex of Saccharomyces cerevisiae.PMID 12966094Read ↗

Analysis on origin recognition complex containing Orc5p with defective Walker A motif.Semantic proximity Read ↗

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Mechanism for the degradation of origin recognition complex containing Orc5p with a defective Walker A motif and its suppression by over-production of Orc4p in yeast cells.Semantic proximity Read ↗

The origin recognition complex in silencing, cell cycle progression, and DNA replication.Semantic proximity Read ↗

Analysis of mutant origin recognition complex with reduced ATPase activity in vivo and in vitro.Semantic proximity Read ↗

ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA.Semantic proximity Read ↗

ATP hydrolysis by ORC catalyzes reiterative Mcm2-7 assembly at a defined origin of replication.Semantic proximity Read ↗

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ATP-dependent pre-replicative complex assembly is facilitated by Adk1p in budding yeast.Semantic proximity Read ↗

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