Article
Analysis of mutant origin recognition complex with reduced ATPase activity in vivo and in vitro.
The Biochemical journal - 1 Aug 2008
Takehara Masaya, Makise Masaki, Takenaka Hitomi, Asano Teita, Mizushima Tohru
Abstract excerpt
In eukaryotes, ORC (origin recognition complex), a six-protein complex, is the most likely initiator of chromosomal DNA replication. ORC belongs to the AAA(+) (ATPases associated with a variety of cellular activities) family of proteins and has intrinsic ATPase activity derived from Orc1p, one of its subunits. To reveal the role of this ATPase activity in Saccharomyces cerevisiae (baker's yeast) ORC, we mutated...
Topics
- Adenosine Triphosphatases
- Arginine
- Asparagine
- Cell Cycle
- Chromatin Immunoprecipitation
- DNA Replication
- DNA, Fungal
- Electrophoretic Mobility Shift Assay
- Flow Cytometry
- Immunoblotting
- Mutation
- Origin Recognition Complex
- Protein Binding
- Saccharomyces cerevisiae Proteins