Studies of the properties of human origin recognition complex and its Walker A motif mutants.

doi:10.1073/pnas.0408690102

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Article

Studies of the properties of human origin recognition complex and its Walker A motif mutants.

Proceedings of the National Academy of Sciences of the United States of America - 4 Jan 2005

Giordano-Coltart Jennifer, Ying Carol Y, Gautier Jean, Hurwitz Jerard

Abstract excerpt

The eukaryotic six-subunit origin recognition complex (ORC) governs the initiation site of DNA replication and formation of the prereplication complex. In this report we describe the isolation of the wild-type Homo sapiens (Hs)ORC and variants containing a Walker A motif mutation in the Orc1, Orc4, or Orc5 subunit using the baculovirus-expression system. Coexpression of all six HsORC subunits yielded a stable...

Topics

Adenosine Triphosphate
Amino Acid Motifs
Animals
Baculoviridae
Cloning, Molecular
DNA Replication
DNA-Binding Proteins
Genetic Vectors
Humans
Mutation
Origin Recognition Complex
Xenopus
Xenopus Proteins

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Identifiers

PMID: 15618391
DOI: 10.1073/pnas.0408690102
PMCID: PMC544074

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