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Article

Open-state disulfide crosslinking between Mycobacterium tuberculosis mechanosensitive channel subunits.

Biophysical journal - 1 Apr 2003

Shapovalov George, Bass Randal, Rees Douglas C, Lester Henry A

Abstract excerpt

The mechanosensitive channel of large conductance from Mycobacterium tuberculosis (Tb-MscL) was subjected to cysteine-scanning mutagenesis at several residues in the M1 region. The V15C channel displayed disulfide crosslinking in air, but not in the presence of 100 mM beta-mercaptoethanol. In single-channel experiments, the V15C channel was more sensitive to tension than was wild-type Tb-MscL. In air, Tb-MscL...

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Identifiers

PMID: 12668444
DOI: 10.1016/S0006-3495(03)75041-3
PMCID: PMC1302802

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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