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Article

Gain-of-function mutations reveal expanded intermediate states and a sequential action of two gates in MscL.

The Journal of general physiology - 1 Feb 2005

Anishkin Andriy, Chiang Chien-Sung, Sukharev Sergei

Abstract excerpt

The tension-driven gating transition in the large mechanosensitive channel MscL proceeds through detectable states of intermediate conductance. Gain-of-function (GOF) mutants with polar or charged substitutions in the main hydrophobic gate display altered patterns of subconducting states, providing valuable information about gating intermediates. Here we present thermodynamic analysis of several GOF mutants to...

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Identifiers

PMID: 15684093
DOI: 10.1085/jgp.200409118
PMCID: PMC2217497

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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