Article
Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity.
Biophysical journal - 1 Oct 1999
Yoshimura K, Batiza A, Schroeder M, Blount P, Kung C
Abstract excerpt
Mechanosensitive channel large (MscL) encodes the large conductance mechanosensitive channel of the Escherichia coli inner membrane that protects bacteria from lysis upon osmotic shock. To elucidate the molecular mechanism of MscL gating, we have comprehensively substituted Gly(22) with all other common amino acids. Gly(22) was highlighted in random mutagenesis screens of E. coli MscL (, Proc. Nat. Acad. Sci....
Topics
- Amino Acid Substitution
- Bacterial Proteins
- Culture Media
- Electric Conductivity
- Escherichia coli
- Escherichia coli Proteins
- Glycine
- Hydrogen-Ion Concentration
- Ion Channel Gating
- Ion Channels
- Kinetics
- Models, Molecular
- Mutation
- Normal Distribution