Article
Generation and evaluation of a large mutational library from the Escherichia coli mechanosensitive channel of large conductance, MscL: implications for channel gating and evolutionary design.
The Journal of biological chemistry - 6 Jun 2003
Maurer Joshua A, Dougherty Dennis A
Abstract excerpt
Random mutagenesis of the mechanosensitive channel of large conductance (MscL) from Escherichia coli coupled with a high-throughput functional screen has provided new insights into channel structure and function. Complementary interactions of conserved residues proposed in a computational model for gating have been evaluated, and important functional regions of the channel have been identified. Mutational...
Topics
- Amino Acid Sequence
- Conserved Sequence
- Escherichia coli
- Escherichia coli Proteins
- Evolution, Molecular
- Gene Library
- Ion Channel Gating
- Ion Channels
- Mechanotransduction, Cellular
- Molecular Sequence Data
- Mutagenesis
- Phenotype
- Protein Structure, Tertiary