Article
Setting of graded levels of a protein in yeast by a t-degron technique as applied to phosphoglycerate mutase.
BMC genetics - 30 Jul 2002
Heidrich Katja, Fraenkel Dan G
Abstract excerpt
BACKGROUND: Setting of graded levels of a protein for in vivo studies by controlled gene expression has inconveniences, and we here explore the use of the t-degron technique instead. RESULTS: In a yeast t-degron (ubiquitin-argDHFR(ts))- phosphoglycerate mutase (GPM1) fusion strain, increasing periods of exposure to the non-permissive temperature 37 degrees C, even in the presence of cycloheximide, gave decreasing...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.