Article
Heat-inducible degron: a method for constructing temperature-sensitive mutants.
Science (New York, N.Y.) - 4 Mar 1994
Dohmen R J, Wu P, Varshavsky A
Abstract excerpt
A temperature-sensitive (ts) mutant retains the function of a gene at a low (permissive) temperature but not at a high (nonpermissive) temperature. Arg-DHFR, a dihydrofolate reductase bearing an amino-terminal (N-terminal) arginine, is long-lived in the yeast Saccharomyces cerevisiae, even though...
Topics
- CDC28 Protein Kinase, S cerevisiae
- Fungal Proteins
- Half-Life
- Hot Temperature
- Mutagenesis
- Phenotype
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Temperature
- Tetrahydrofolate Dehydrogenase
- Ubiquitins