Article

Degradation Bottlenecks and Resource Competition in Transiently and Stably Engineered Mammalian Cells

2024-06-03

Abstract excerpt

Degradation tags, otherwise known as degrons, are portable sequences that can be used to alter protein stability. Here, we report that degron-tagged proteins compete for cellular degradation resources in engineered mammalian cells leading to coupling of the degradation rates of otherwise independently expressed proteins when constitutively targeted human degrons are adopted. By adopting inducible bacterial and pla...

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Literature Corpus work: abed4598-563d-51bc-a2af-e7d617476efd
DOI: 10.1101/2024.06.03.597247

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