Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Generic tools for conditionally altering protein abundance and phenotypes on demand.

Biological chemistry - 1 Jul 2014

Faden Frederik, Mielke Stefan, Lange Dieter, Dissmeyer Nico

Abstract excerpt

Conditional gene expression and modulating protein stability under physiological conditions are important tools in biomedical research. They led to a thorough understanding of the roles of many proteins in living organisms. Current protocols allow for manipulating levels of DNA, mRNA, and of functional proteins. Modulating concentrations of proteins of interest, their post-translational processing, and their...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA
Share to Phenotype
Share to Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to RNA, Messenger

Source

Identifiers

PMID: 25003383
DOI: 10.1515/hsz-2014-0160

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Generic tools for conditionally altering protein abundance and phenotypes on demand.PMID 25003383Read ↗

Phenotypes on demand via switchable target protein degradation in multicellular organisms.Semantic proximity Read ↗

Targeted protein depletion in Saccharomyces cerevisiae by activation of a bidirectional degron.Semantic proximity Read ↗

Efficient protein depletion by genetically controlled deprotection of a dormant N-degron.Semantic proximity Read ↗

Genetic manipulation and targeted protein degradation in mammalian systems: practical considerations, tips and tricks for discovery research.Semantic proximity Read ↗

Comparative analysis and directed protein evolution yield an improved degron technology with minimal basal degradation, rapid inducible depletion, and faster recovery of target proteins.Semantic proximity Read ↗

Manipulating gene expression levels in mammalian cell factories: An outline of synthetic molecular toolboxes to achieve multiplexed controlSemantic proximity Read ↗

Setting of graded levels of a protein in yeast by a t-degron technique as applied to phosphoglycerate mutase.Semantic proximity Read ↗

Inducible degron and its application to creating conditional mutants.Semantic proximity Read ↗

A toolbox for conditional control of gene expression in apicomplexan parasitesSemantic proximity Read ↗

Engineering the auxin-inducible degron system for tunable <i>in vivo</i> control of organismal physiologySemantic proximity Read ↗

Degradation Bottlenecks and Resource Competition in Transiently and Stably Engineered Mammalian CellsSemantic proximity Read ↗

5’Untranslated regions provide a versatile toolkit for tunable exogenous protein expressionSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.