Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Growth-based determination and biochemical confirmation of genetic requirements for protein degradation in Saccharomyces cerevisiae.

Journal of visualized experiments : JoVE - 16 Feb 2015

Watts Sheldon G, Crowder Justin J, Coffey Samuel Z, Rubenstein Eric M

Abstract excerpt

Regulated protein degradation is crucial for virtually every cellular function. Much of what is known about the molecular mechanisms and genetic requirements for eukaryotic protein degradation was initially established in Saccharomyces cerevisiae. Classical analyses of protein degradation have re...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Blotting, Western
Share to Mutation
Share to Proteolysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Saccharomyces cerevisiae

Share to Saccharomyces cerevisiae Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 25742191
DOI: 10.3791/52428
PMCID: PMC4354639

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-25742191-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Growth-based determination and biochemical confirmation of genetic requirements for protein degradation in Saccharomyces cerevisiae.PMID 25742191Read ↗

A proteome-wide yeast degron collection for the dynamic study of protein function.Semantic proximity Read ↗

Targeted protein depletion in Saccharomyces cerevisiae by activation of a bidirectional degron.Semantic proximity Read ↗

Yeast as a model to study protein misfolding in aged cellsSemantic proximity Read ↗

Endoplasmic reticulum-associated protein quality control and degradation: screen for ERAD mutants after ethylmethane sulfonate mutagenesis.Semantic proximity Read ↗

A genomic screen identifies Dsk2p and Rad23p as essential components of ER-associated degradation.Semantic proximity Read ↗

Growth assays to assess polyglutamine toxicity in yeast.Semantic proximity Read ↗

Engineering stringent genetic biocontainment of yeast with a protein stability switchSemantic proximity Read ↗

Stress tolerance of misfolded carboxypeptidase Y requires maintenance of protein trafficking and degradative pathways.Semantic proximity Read ↗

Genetic Selection Based on a Ste6*C-HA-Ura3 Substrate Identifies New Cytosolic Quality Control Alleles in Saccharomyces cerevisiae.Semantic proximity Read ↗

High-throughput analysis of in vivo protein stability.Semantic proximity Read ↗

Exploration of essential gene functions via titratable promoter alleles.Semantic proximity Read ↗

Saccharomyces cerevisiae as a biotechnological tool for ageing research : studies on translation and metabolismSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.