Article
Analysis of a conditional degradation signal in yeast and mammalian cells.
European journal of biochemistry - 1 Jan 1999
Lévy F, Johnston J A, Varshavsky A
Abstract excerpt
The N-end rule pathway is a ubiquitin-dependent proteolytic system, the targets of which include proteins that bear destabilizing N-terminal residues. The latter are a part of the degradation signal called the N-degron. Arg-DHFRts, an engineered N-end rule substrate, bears N-terminal arginine (a...
Topics
- Animals
- Arginine
- Endopeptidases
- Folic Acid Antagonists
- Methotrexate
- Mice
- Models, Biological
- Multienzyme Complexes
- Mutation
- Recombinant Proteins
- Saccharomyces cerevisiae
- Species Specificity
- Substrate Specificity
- Tetrahydrofolate Dehydrogenase
- Thymidylate Synthase
- Ubiquitins