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Article

Inducible degron and its application to creating conditional mutants.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006

Dohmen R Jürgen

Abstract excerpt

Conditional mutants are important tools particularly in the analysis of essential genes. In this chapter, a method is described that allows for a rapid design-based generation of temperature-sensitive alleles of many Saccharomyces cerevisiae genes. The method employs a temperature-inducible degron, denoted as td, which, when linked to the N-terminus of proteins to be studied, targets them for rapid degradation...

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PMID: 16118432
DOI: 10.1385/1-59259-958-3:145

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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