Article
In vitro induction of somatic mutations in human peripheral B lymphocytes.
International journal of molecular medicine - 1 Oct 2000
Zafiropoulos A, Kandilogiannaki M, Dahlenborg C, Borrebaeck C A, Krambovitis E
Abstract excerpt
The major cellular and molecular factors that lead to the induction of somatic mutations in human B cells remain unclear. This study describes an approach which allowed us to single-cell culture antigen-specific B cells after in vitro immunization. Using single strand conformational polymorphism analysis on the descendant cultures we were able to demonstrate that the Ig gene of B cells was induced to mutate....
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