Article
TdT-accessible breaks are scattered over the immunoglobulin V domain in a constitutively hypermutating B cell line.
Immunity - 1 Dec 1998
Sale J E, Neuberger M S
Abstract excerpt
Searching for an in vitro model for somatic hypermutation, we have identified an IgM-expressing Burkitt lymphoma line that constitutively diversifies its immunoglobulin V domain at high rate during culture. As in in vivo, the mutations are largely nucleotide substitutions with the pattern of subs...
Topics
- B-Lymphocytes
- DNA Damage
- DNA Nucleotidylexotransferase
- Humans
- Immunoglobulin Constant Regions
- Immunoglobulin Heavy Chains
- Immunoglobulin M
- Immunoglobulin Variable Region
- Immunoglobulin lambda-Chains
- Immunoglobulin mu-Chains
- Mutagenesis, Insertional
- Mutation
- Phenotype
- Tumor Cells, Cultured