Article
In vitro triggering of somatic mutation in human naive B cells.
Journal of immunology (Baltimore, Md. : 1950) - 1 Oct 1997
Razanajaona D, Denépoux S, Blanchard D, de Bouteiller O, Liu Y J, Banchereau J, Lebecque S
Abstract excerpt
During T cell-dependent immune response, germinal center B cells accumulate somatic mutations in their Ig V(D)J genes and give rise to affinity-selected B cells. We tested several culture conditions for triggering somatic mutation in human tonsillar naive slgD+CD23+ cells after cross-linking thei...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- B-Lymphocytes
- CD3 Complex
- Cell Culture Techniques
- Clone Cells
- Coculture Techniques
- Genes, Immunoglobulin
- Humans
- Immunoglobulin D
- Immunoglobulin Isotypes
- Immunoglobulin Variable Region
- Interphase
- Lymphocyte Activation
- Molecular Sequence Data
- Mutation
- Palatine Tonsil
- Receptors, Antigen, B-Cell