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Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling

2023-11-17

Abstract excerpt

<h4>ABSTRACT</h4> Engineering bacterial metabolism to efficiently produce chemicals and materials from multi-step pathways requires optimizing multi-gene expression programs to achieve enzyme balance. CRISPR-Cas transcriptional control systems are emerging as important metabolic engineering tools for programming multi-gene expression regulation. However, poor predictability of guide RNA folding can disrupt enzyme...

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Literature Corpus work
ff96fc56-a8c8-57ba-a6aa-e70f24e91779
DOI
10.1101/2023.11.17.567465
Open publication

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Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profilingDOI 10.1101/2023.11.17.567465
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