Article

Optimization of Cas12a for multiplexed genome-scale transcriptional activation

2023-04-13

Abstract excerpt

<h4>ABSTRACT</h4> Cas12a CRISPR technology, unlike Cas9, allows for facile multiplexing of guide RNAs from a single transcript, simplifying combinatorial perturbations. While Cas12a has been implemented for multiplexed knockout genetic screens, it has yet to be optimized for CRISPR activation (CRISPRa) screens in human cells. Here we develop a new Cas12a-based transactivation domain (TAD) recruitment system using...

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Literature Corpus work: 69ae08a4-cd30-5835-9906-8528210fb79e
DOI: 10.1101/2023.04.13.536783

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