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Article

Selection of chromosomal DNA libraries using a multiplex CRISPR system.

eLife - 19 Aug 2014

Ryan Owen W, Skerker Jeffrey M, Maurer Matthew J, Li Xin, Tsai Jordan C, Poddar Snigdha, Lee Michael E, DeLoache Will, Dueber John E, Arkin Adam P, Cate Jamie H D

Abstract excerpt

The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number effects, or the use of complex marker-dependent chromosomal integration strategies. We developed quantitative gene assembly and DNA...

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Identifiers

PMID: 25139909
DOI: 10.7554/eLife.03703
PMCID: PMC4161972

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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