Pooled CRISPR screening enables genotype-phenotype association in industrial microorganisms.

Abstract excerpt

Industrial microorganisms combine genomic robustness with process resilience to achieve high-level bioproduction, and these traits are usually governed by complex, multi-gene interactions. Pooled CRISPR screening-assisted genotype-phenotype association (GPA), an emerging approach attracting increasing attention, has recently evolved into a powerful platform for systematically interrogating gene function in...

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