Article

Principles for rational Cas13d guide design

2019-12-28

Abstract excerpt

Type VI CRISPR enzymes have recently been identified as programmable RNA-guided, RNA-targeting Cas proteins with nuclease activity that allow for specific and robust target gene knock-down without altering the genome. However, we currently lack information about optimal Cas13 guide RNA designs for high target RNA knock-down efficacy. To close this gap, we conducted four massively-parallel Cas13 screens targeting t...

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Literature Corpus work: e0d2d060-ece5-5bc1-b7ce-ad3bc31e9532
DOI: 10.1101/2019.12.27.889089

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