Specific Modulation of CRISPR Transcriptional Activators through RNA-Sensing Guide RNAs in Mammalian Cells and Zebrafish Embryos

Abstract excerpt

Cellular transcripts encode important information regarding cell identity and disease status. The activation of CRISPR in response to RNA biomarkers holds the potential for controlling CRISPR activity with spatiotemporal precision. This would enable the restriction of CRISPR activity to specific cell types expressing RNA biomarkers of interest while preventing unwanted activity in other cells. Here, we present a s...

Topics

Open a Topic to create a Post that cites this publication.

• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR and Genetic Engineering
• CRISPR-Associated Proteins
• CRISPR-Cas Systems
• Directed Molecular Evolution
• Nucleotide Motifs
• Protein Engineering
• Staphylococcus aureus

Identifiers and source

Literature Corpus work

9e5c8c51-72da-5f26-9c05-509e092b9cf0

DOI

10.1101/2023.05.08.539738