Article

Experimental Test of Evolutionary Safety of a CRISPR-Cas9 Gene-Drive Element

2023-11-29

Abstract excerpt

CRISPR-Cas9 gene drives (CCGDs) are powerful tools for genetic control of wild populations, with applications from disease eradication to species conservation. However, Cas9 alone and in a complex with gRNA can cause double-stranded DNA breaks at off-target sites, which could increase the mutational load and lead to unintended loss-of-heterozygosity (LOH) events. These undesired effects raise potential concerns ab...

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Literature Corpus work: fa81d028-cd4e-5597-b890-bad977834dc3
DOI: 10.1101/2023.11.28.569142

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