Article

CRISPR-Cas9 editing induces Loss of Heterozygosity in the pathogenic yeast <i>Candida parapsilosis</i>

2022-08-16

Abstract excerpt

<h4>ABSTRACT</h4> Genetic manipulation is often used to study gene function. However, unplanned genome changes (including Single Nucleotide Polymorphisms (SNPs), aneuploidy and Loss of Heterozygosity (LOH)) can affect the phenotypic traits of the engineered strains. Here, we show that CRISPR-Cas9 editing can induce LOH in the diploid human pathogenic yeast Candida parapsilosis . We sequenced the genomes of ten i...

Identifiers and source

Literature Corpus work: e700ce20-4b10-5fa4-b2e5-a55963291694
DOI: 10.1101/2022.08.15.504067

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CRISPR-Cas9 editing induces Loss of Heterozygosity in the pathogenic yeast <i>Candida parapsilosis</i>DOI 10.1101/2022.08.15.504067Read

Targeted loss of heterozygosity in <i>Candida albicans</i> using CRISPR-Cas9Semantic proximity Read

Plasmid-based CRISPR-Cas9 gene editing in multiple <i>Candida</i> speciesSemantic proximity Read

Targeted loss of heterozygosity in Candida albicans using CRISPR-Cas9 reveals the functional impact of allelic variation.Semantic proximity Read

Gene editing in clinical isolates of Candida parapsilosis using CRISPR/Cas9Semantic proximity Read

Allele-specific genome editing using CRISPR-Cas9 causes off-target mutations in diploid yeastSemantic proximity Read

The Genome of the Human Pathogen <i>Candida albicans</i> is Shaped by Mutation and Cryptic Sexual RecombinationSemantic proximity Read

Analysis of Repair Mechanisms following an Induced Double-Strand Break Uncovers Recessive Deleterious Alleles in the Candida albicans Diploid Genome.Semantic proximity Read

Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida SpeciesSemantic proximity Read

Small pangenome of <i>Candida parapsilosis</i> reflects overall low intraspecific diversitySemantic proximity Read

Global analysis of mutations driving microevolution of a heterozygous diploid fungal pathogen.Semantic proximity Read

The CRISPR toolbox in medical mycology: State of the art and perspectivesSemantic proximity Read

The diploid genome sequence of <i>Candida albicans</i>Semantic proximity Read