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Article

Highly parallel genome variant engineering with CRISPR-Cas9.

Nature genetics - 1 Apr 2018

Sadhu Meru J, Bloom Joshua S, Day Laura, Siegel Jake J, Kosuri Sriram, Kruglyak Leonid

Abstract excerpt

Understanding the functional effects of DNA sequence variants is of critical importance for studies of basic biology, evolution, and medical genetics; however, measuring these effects in a high-throughput manner is a major challenge. One promising avenue is precise editing with the CRISPR-Cas9 system, which allows for generation of DNA double-strand breaks (DSBs) at genomic sites matching the targeting sequence...

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Identifiers

PMID: 29632376
DOI: 10.1038/s41588-018-0087-y
PMCID: PMC6115196

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