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A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast.

BMC genomics - 23 Mar 2021

McGlincy Nicholas J, Meacham Zuriah A, Reynaud Kendra K, Muller Ryan, Baum Rachel, Ingolia Nicholas T

Abstract excerpt

BACKGROUND: CRISPR/Cas9-mediated transcriptional interference (CRISPRi) enables programmable gene knock-down, yielding loss-of-function phenotypes for nearly any gene. Effective, inducible CRISPRi has been demonstrated in budding yeast, and genome-scale guide libraries enable systematic, genome-wide genetic analysis. RESULTS: We present a comprehensive yeast CRISPRi library, based on empirical design rules,...

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Clustered Regularly Interspaced Short Palindromic Repeats
CRISPR-Cas Systems
Phenotype
RNA, Guide, CRISPR-Cas Systems
Saccharomyces cerevisiae

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PMID: 33757429
DOI: 10.1186/s12864-021-07518-0
PMCID: PMC7986282

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