Article

Allele-specific genome editing using CRISPR-Cas9 causes off-target mutations in diploid yeast

2018-08-22

Abstract excerpt

<h4>ABSTRACT</h4> Targeted DNA double-strand breaks (DSBs) with CRISPR-Cas9 have revolutionized genetic modification by enabling efficient genome editing in a broad range of eukaryotic systems. Accurate gene editing is possible with near-perfect efficiency in haploid or (predominantly) homozygous genomes. However, genomes exhibiting polyploidy and/or high degrees of heterozygosity are less amenable to genetic mod...

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Literature Corpus work: d6d6cf63-a487-573b-a30c-4213802aec58
DOI: 10.1101/397984

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