Upper bound on the mutational burden imposed by a CRISPR-Cas9 gene-drive element.

Abstract excerpt

Homing-based CRISPR-Cas9 gene drives (CCGDs) are powerful tools for genetic control of wild populations, with applications from disease eradication to species conservation. However, Cas9 alone and in a complex with guide RNA can cause double-stranded DNA breaks at off-target sites, which could increase the mutational load and lead to unintended loss-of-heterozygosity (LOH) events. These undesired effects raise...

Topics

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• CRISPR-Cas Systems
• Loss of Heterozygosity
• Mutation
• Mutation Rate
• Saccharomyces cerevisiae