Article

Higher resolution pooled genome-wide CRISPR knockout screening in Drosophila cells using Integration and Anti-CRISPR (IntAC)

2024-09-19

Abstract excerpt

CRISPR screens enable systematic, scalable genotype-to-phenotype mapping. We previously developed a pooled CRISPR screening method for Drosophila melanogaster and mosquito cell lines using plasmid transfection and site-specific integration to introduce single guide (sgRNA) libraries, followed by PCR and sequencing of integrated sgRNAs. While effective, the method relies on early constitutive Cas9 activity that po...

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Literature Corpus work: f77a08cb-6f80-5aa5-a1f5-cb494e1b1db9
DOI: 10.1101/2024.09.19.613976

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