Article

Harnessing anti-CRISPR to suppress, subtract, and segregate Cas9 activity for precision CRISPR in <i>Drosophila</i>

2025-12-09

Abstract excerpt

<h4>ABSTRACT</h4> Tissue-specific CRISPR (ts-CRISPR) is a powerful approach for studying cell and developmental biology by restricting mutagenesis to specific tissues. However, the precision of this approach is often compromised by non-specific, “leaky” Cas9 activity that confounds phenotypic analysis and destabilizes Cas9/gRNA stocks. To address these limitations in Drosophila , we developed a toolkit based on...

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Literature Corpus work: 2697e7b2-d0db-5cf6-a932-5ac091d943d6
DOI: 10.64898/2025.12.07.692848

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