Article

Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in <i>Drosophila cells</i>

2018-03-01

Abstract excerpt

Genome-wide screens in Drosophila cells have offered numerous insights into gene function, yet a major limitation has been the inability to stably deliver large multiplexed DNA libraries to cultured cells allowing barcoded pooled screens. Here, we developed a site-specific integration strategy for library delivery and performed a genome-wide CRISPR knockout screen in Drosophila S2R+ cells. Under basal growth con...

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Literature Corpus work: 9a984001-a535-5983-9e64-5834b639b5e0
DOI: 10.1101/274464

Open publication

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