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Article

Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells.

eLife - 27 Jul 2018

Viswanatha Raghuvir, Li Zhongchi, Hu Yanhui, Perrimon Norbert

Abstract excerpt

Genome-wide screens in Drosophila cells have offered numerous insights into gene function, yet a major limitation has been the inability to stably deliver large multiplexed DNA libraries to cultured cells allowing barcoded pooled screens. Here, we developed a site-specific integration strategy for library delivery and performed a genome-wide CRISPR knockout screen in Drosophila S2R+ cells. Under basal growth...

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Identifiers

PMID: 30051818
DOI: 10.7554/eLife.36333
PMCID: PMC6063728

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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